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[DNA-like duplexes containing repetitive sequences. VIII. Synthesis and properties of DNA fragments--substrates of restriction endonuclease EcoRII]статья
Дата последнего поиска статьи во внешних источниках: 1 апреля 2020 г.
- Авторы: Gromova E.S., Vinogradova M.N., Elov A.E., Veĭko V.P., Dolinnaia N.G.
- Журнал: Molekuliarnaia biologiia
- Том: 18
- Номер: 2
- Год издания: 1984
- Издательство: Russian Academy of Sciences
- Местоположение издательства: Russian Federation
- Первая страница: 370
- Последняя страница: 381
- Аннотация: The optimal way of constructing a family of restriction endonuclease EcoRII substrates has been developed. The substrates are DNA-like duplexes containing regularly repeated native or modified sites of this enzyme as well as those of EcoRI and AluI. Synthesis of substrates was performed by water-soluble carbodiimide-induced polycondensation of two nonanucleotides, d(C-C-T-G-G-A-A-T-Tp) and d(C-C-A-G-G-A-G-C-Tp), as constituents of different complementary complexes. The products of reaction (degree of polymerization, 2-20) were isolated by G-200 gel-filtration. The yield of polymers was about 70%. The main products of reaction were dimers when dephosphorylated nonanucleotides (terminators of polycondensation) were used. The thermal stability of DNA-like duplexes is very high. The structure of the polymers obtained has been confirmed by UV-spectroscopy and by CD data as well as by the results of cleavage by EcoRI and AluI restriction endonucleases.
- Добавил в систему: Вейко Владимир Петрович