A single mutation converts bacterial Na+-transporting rhodopsin into an H+ transporterстатья
Статья опубликована в высокорейтинговом журнале
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Дата последнего поиска статьи во внешних источниках: 12 июля 2019 г.
Аннотация:Na+-rhodopsins are light–driven pumps used by marine bacteria to extrude Na+ ions from the cytoplasm. We show here that replacement of Gln123 on the cytoplasmic side of the ion–conductance channel with aspartate or glutamate confers H+ transport activity to the Na+-rhodopsin from Dokdonia sp. PRO95. The Q123E variant could transport H+ out of Escherichia coli cells in the medium containing 100 mM Na+ and SCN– as the penetrating anion. The rates of the photocycle steps of this variant were only marginally dependent on Na+, and the major electrogenic steps were the decays of the K and O intermediates.