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Reactivation of Dyen chromosome upon reprogramming leads to changes in the replication pattern and 5hmC accumulationстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 26 августа 2016 г.
- Авторы: Bogomazova Alexandra N., Lagarkova Maria A., Panova Alexandra V., Nekrasov Evgueny D., Kiselev Sergey L.
- Журнал: Chromosoma
- Том: 123
- Номер: 1-2
- Год издания: 2014
- Издательство: Springer Verlag
- Местоположение издательства: Germany
- Первая страница: 117
- Последняя страница: 128
- DOI: 10.1007/s00412-013-0433-x
- Аннотация: Once set, the inactive status of the X chromosome in female somatic cells is preserved throughout subsequent cell divisions. The inactive status of the X chromosome is characterized by many features, including late replication. In contrast to induced pluripotent stem cells (iPSCs) in mice, the X chromosome in human female iPSCs usually remains inactive after reprogramming of somatic cells to the pluripotent state, although recent studies point to the possibility of reactivation of the X chromosome. Here, we demonstrated that, during reprogramming, the inactive X chromosome switches from late to synchronous replication, with restoration of the transcription of previously silenced genes. This process is accompanied by accumulation of a new epigenetic mark or intermediate of the DNA demethylation pathway, 5-hydroxymethylcytosine (5hmC), on the activated X chromosome. Our results indicate that the active status of the X chromosome is better confirmed by early replication and the reappearance of 5hmC, rather than by appearance of histone marks of active chromatin, removal of histone marks of inactive chromatin, or an absence of XIST coating.
- Добавил в систему: Лагарькова Мария Андреевна