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NMDA-receptors are involved in Cu2+/paraquat-induced death of cultured cerebellar granule neuronsстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 2 ноября 2016 г.
- Авторы: Stelmashook E.V., Genrikhs E.E., Aleksandrova O.P., Amelkina G.A., Zelenova E.A., Isaev N.K.
- Журнал: Biochemistry (Moscow)
- Том: 81
- Номер: 8
- Год издания: 2016
- Издательство: Pleiades Publishing, Ltd
- Местоположение издательства: Road Town, United Kingdom
- Первая страница: 899
- Последняя страница: 905
- DOI: 10.1134/s0006297916080113
- Аннотация: Rat cultured cerebellar granule neurons (CGNs) were not sensitive to CuCl2 (1-10 μM, 24 h), whereas paraquat (150 μM) decreased neuronal survival to 79 ± 3% of control level. Simultaneous treatment of CGNs with paraquat and CuCl2 (2, 5, or 10 μM Cu2+/paraquat) caused significant copper dose-dependent death, lowering their survival to 56 ± 4, 37 ± 3, or 16 ± 2%, respectively, and stimulating elevated production of free radicals in CGNs. Introduction of vitamin E, a noncompetitive antagonist of NMDA subtype of glutamate receptor (MK-801), and also removal of glutamine from the incubation medium decreased toxicity of Cu2+/paraquat mixture. However, addition of Cu2+ into the incubation medium did not affect CGNs death caused by glutamate. These data emphasize that excessive copper in the brain may trigger oxidative stress, which in turn results in release of glutamate, overstimulation of glutamate receptors, and neuronal death.
- Добавил в систему: Александрова Ольга Петровна