Lipid dynamics in nanoparticles formed by maleic acid-containing copolymers: EPR spectroscopy and molecular dynamics simulations

Colbasevici, A.; Voskoboynikova, N.; Orekhov, P.S.; Bozdaganyan, M.E.; Karlova, M.G.; Sokolova, O.S.; Klare, J.P.; Mulkidjanian, A.Y.; Shaitan, K.V.; Steinhoff, H.

Статья опубликована в высокорейтинговом журнале

Информация о цитировании статьи получена из Web of Science, Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 3 июня 2020 г.

Авторы: Colbasevici Alexandr, Voskoboynikova Natalia, Orekhov Philipp S., Bozdaganyan Marine E., Karlova Maria G., Sokolova Olga S., Klare Johann P., Mulkidjanian Armen Y., Shaitan Konstantin V., Steinhoff Heinz-Jürgen
Журнал: Biochimica et Biophysica Acta - Biomembranes
Год издания: 2020
Издательство: Elsevier BV
Местоположение издательства: Netherlands
Первая страница: 183207
DOI: 10.1016/j.bbamem.2020.183207
Аннотация: Amphiphilic maleic acid-containing copolymers account for a recent methodical breakthrough in the study of membrane proteins. Their application enables a detergent-free extraction of membrane proteins from lipid bilayers, yielding stable water-soluble, discoidal lipid bilayer particles with incorporated proteins, which are wrapped with copolymers. Although many studies confirm the potential of this approach for membrane protein research, the interactions between the maleic acid-containing copolymers and extracted lipids, as well as possible effects of the copolymers on lipid-embedded proteins deserve further scrutinization. Here, we combine electron paramagnetic resonance spectroscopy and coarse-grain molecular dynamics simulations to compare the distribution and dynamics of lipids in lipid particles of phospholipid bilayers encased either by an aliphatic diisobutylene/maleic acid copolymer (DIBMALPs) or by an aromatic styrene/maleic acid copolymer (SMALPs). Nitroxides located at the 5th, 12th or 16th carbon atom positions in phosphatidylcholine-based spin labels experience restrictions of their reorientational motion depending on the type of encasing copolymer. The dynamics of the lipids was less constrained in DIBMALPs than in SMALPs with the affinity of spin labeled lipids to the polymeric rim being more pronounced in SMALPs.
Добавил в систему: Боздаганян Маринэ Евгеньевна

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Lipid dynamics in nanoparticles formed by maleic acid-containing copolymers: EPR spectroscopy and molecular dynamics simulationsстатья Исследовательская статья

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