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Physicochemical characteristics of human IgG Fc fragments that expose regulatory rheumatoid factor neoepitopes and may show promise as anti-rheumatic agentsстатья Исследовательская статья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 23 декабря 2020 г.
- Авторы: Sidorov Alexandr, Beduleva Liubov, Menshikov Igor, Terentiev Alexey, Cherepanov Igor
- Журнал: Biotechnology and Applied Biochemistry
- Том: 67
- Номер: 2
- Год издания: 2020
- Издательство: Portland Press, Ltd.
- Местоположение издательства: United Kingdom
- Первая страница: 287
- Последняя страница: 293
- DOI: 10.1002/bab.1849
- Аннотация: Previously, we showed that IgG Fc fragments can expose neoepitopes specific to antibodies that were named regulatory rheumatoid factor (regRF). RegRF confers resistance to experimental autoimmune diseases. Immunization of rats with rat Fc fragments exposing neoepitopes recognized by regRF reduces the symptoms of collagen-induced arthritis. Therefore, IgG Fc fragments that expose neoepitopes recognized by regRF are promising anti-rheumatic agents and regRF-producing lymphocytes are potential therapeutic biotargets. The purpose of this study was to elucidate the physicochemical features of human IgG Fc fragments that are associated with the presence of immunosuppressive neoepitopes recognized by regRF. It was found that the acquisition of neoepitopes recognized by regRF is associated with a reduction of the hinge disulfide bonds and conformational changes in the Fc fragment domains. Alkylation of thiol groups in the hinge leads to loss of the epitopes. Therefore the neoepitopes recognized by regRF may form directly in the hinge when interchain disulfide bonds are reduced or in the region of the CH2 domain as part of the conformational changes caused by the reduction of the interchain disulfide bonds in the hinge. Species-specificity studies of neoepitopes recognized by regRF revealed that human and rat neoepitopes recognized by regRF are cross-reactive. This article is protected by copyright. All rights reserved.
- Добавил в систему: Бедулева Любовь Викторовна