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Development of anti-zearalenone monoclonal antibody and detection of zearalenone in corn products from China by ELISAстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 19 июля 2013 г.
- Авторы: Shi-Chun Pei, Won-Jong Lee, Gai-Ping Zhang, Xiao-Fei Hu, Eremin Sergei A., Li-Jun Zhang
- Журнал: Food Control
- Том: 31
- Номер: 1
- Год издания: 2013
- Издательство: Elsevier BV
- Местоположение издательства: Netherlands
- Первая страница: 65
- Последняя страница: 70
- DOI: 10.1016/j.foodcont.2012.09.006
- Аннотация: High-throughput screening enzyme-linked immunosorbent assay (HTS-ELISA) method was used for screening of hybridoma cells that secret high-affinity anti-zearalenone (ZON) monoclonal antibody (mAb). Recovery rate of ZON from spiked corn samples was between 80% and 128%. The cross-reactivities of the anti-ZON mAb against ZON, b-zearalenol, a-zearalanol, b-zearalanol, deoxynivalenol and aflatoxin B1 were 100%, 24.1%, 189.1%, 43.9%, <1.0%, <1.0%, respectively. The limit of detection for ZON was 0.1 ng/g. The linear range for the developed indirect competitive ELISA was 0.5e10 ng/g. The half maximal (50%) inhibitory concentration (IC50) of the anti-ZON mAb was 1.79 ng/g. In total, 137 samples of corn, powdered corn, and processed corn products were collected from farms and supermarkets in northeastern China. Of 42 corn samples tested, 4 (9.5%) samples contained ZON 5e60 ng/g, 4 (9.5%) samples contained 60e500 ng/g, In 34 (81%) samples ZON was not detected. Of 15 powered corn samples, only 1 (6.7%) sample contained 5e60 ng/g, and no ZON was detected in 80 processed corn products.
- Добавил в систему: Еремин Сергей Александрович