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Genomic Characterization of Candidate Division LCP-89 Reveals an Atypical Cell Wall Structure, Microcompartment Production, and Dual Respiratory and Fermentative Capacitiesстатья
Статья опубликована в высокорейтинговом журнале
Статья опубликована в журнале из списка Web of Science и/или Scopus- Авторы: Youssef Noha H., Farag Ibrahim F., Ryan Hahn C., Jarett Jessica, Becraft Eric, Eloe-Fadrosh Emiley, Lightfoot Jorge, Bourgeois Austin, Cole Tanner, Ferrante Stephanie, Truelock Mandy, Marsh William, Jamaleddine Michael, Ricketts Samantha, Simpson Ronald, McFadden Allyson, Hoff Wouter, Ravin Nikolai V., Sievert Stefan, Stepanauskas Ramunas, Woyke Tanja, Elshahed Mostafa
- Журнал: Applied and Environmental Microbiology
- Том: 85
- Номер: 10
- Год издания: 2019
- Издательство: American Society for Microbiology
- Местоположение издательства: United States
- DOI: 10.1128/aem.00110-19
- Аннотация: Our understanding of the metabolic capacities, physiological preferences, and ecological roles of yet-uncultured microbial phyla is expanding rapidly. Two distinct approaches are currently being utilized for characterizing microbial communities in nature: amplicon-based 16S rRNA gene surveys for community characterization and metagenomics/single-cell genomics for detailed metabolic reconstruction. The occurrence of multiple yet-uncultured bacterial phyla has been documented using 16S rRNA surveys, and obtaining genome representatives of these yet-uncultured lineages is critical to our understanding of the role of yet-uncultured organisms in nature. This study provides a genomics-based analysis highlighting the structural features and metabolic capacities of a yet-uncultured bacterial phylum (LCP-89) previously identified in 16S rRNA surveys for which no prior genomes have been described. Our analysis identifies several interesting structural features for members of this phylum, e.g., lack of peptidoglycan biosynthetic machinery and the ability to form bacterial microcompartments. Predicted metabolic capabilities include degradation of a wide range of sugars, anaerobic respiratory capacity, and fermentative capacities. In addition to the detailed structural and metabolic analysis provided for candidate division LCP-89, this effort represents an additional step toward a unified scheme for microbial taxonomy by reconciling 16S rRNA gene-based and genomics-based taxonomic outlines.
- Добавил в систему: Равин Николай Викторович