STUDY OF THE INFLUENCE OF BIOLOGICALLY ACTIVE ADDITIVE USING MICROALGAE ON THE GROWTH AND MICROFLORA OF RAINBOW  TROUT

Zayadan, B.K.; Akmukhanova, N.R.; Matorin, D.N.; Sadvakasova, A.K.; Torekhanova, М.М.; Bolatkhan, К.; Balouch, H.; Bauyenova, M.O.

Авторы: Zayadan B.K., Akmukhanova N.R., Matorin D.N., Sadvakasova A.K., Torekhanova М.М., Bolatkhan К., Balouch H., Bauyenova M.O.
Журнал: News of the National Academy of Sciences of the Republic of Kazakhstan, SERIES OF BIOLOGICAL AND MEDICAL
Том: 3
Номер: 333
Год издания: 2019
Первая страница: 39
Последняя страница: 47
DOI: 10.32014/2019.2519-1629.29
Аннотация: Abstract. The aim of this work was to study the effect of biologically active feed additive based on microalgae on the growth parameters and microflora of fish.It was found that the growth rate of fish is directly depended on the amount of chlorella suspension in the feed. Higher length and weight measurements of trout were observed in the variant with the addition of a chlorella suspension in the volume of 10 ml/kg compared to the other variants of our experiment. The variant grown with the addition of chlorella suspension in the amount of 10 ml/kg, had greater increase in weight by 112.7, than the control and experimental variants grown with the addition of chlorella sus- pension in the amount of 5 and 15 ml/kg, showing indicators of 98.2, 97.5, and 99.6, respectively.The survival of fish during the study period remained at a high level (100 %) in the second experimental variant .Survival rate in the control group was reported 97 % and the first variant showed 97.7 %. Despite a significantly high growth rate, the survival rate of fish was amounted to 96.5% in the third variant, Perhaps this is due to the fact that in conditions of excessive intake of chlorella suspension into the environment and with its incomplete assimilation by fish, there is a high reproduction of microalgae in the aquatic environment. Quantitative and qualitative analysis of rainbow trout microflora was carried out in control and experimental variants.The calculation of TMC (full abbreviation) in the bodies of trout in the control variant showed that the number of bacteria ranging from 2,1105 to 3,8105CFU/g in the intestine.Bacterial contamination of the liver was insignificant, and the omch (full abbreviation) index is not more than 1102 КОЕCFU/g wet weight.Gill contamination ranged from 2,1104to 3105 CFU/g. In the experi- mental variants with the addition of Chlorella vulgaris suspension, the omch indices of different trout organs did not differ significantly from those of the control variant. In the intestine, the number of bacteria ranges from 3,1105 to 3,8105 CFU /g, in the liver no more than 2,6102CFU /g wet weight. The insemination of gills was reported from 3105 to 5105 CFU /g. The selected associations of microorganisms consist of both saprophytic and opportunistic bacteria. The studies found that the qualitative composition of the microbiocenosis of trout in both control and experimental versions/variants is represented by 4 main genera Lactobacillus, Aeromonas, Pseudomonas and Acine- tobacter. But the numerical ratio of the bacterium of the genus Lactobacillus was much higher in the experimental variants, in particular in the second variant of the experiment, compared with the control and other variants of the experiment.In addition to the representatives of these genera, representatives of the genera Micrococcus, Sarcina, Bacillus, Enterobacter, Escherichia, Serratia, Proteus and Vibrio were alsofound in different proportions in the trout microflora.The results obtained in the course of the studies allow us to recommend the introduction/addition of feed additives based on microalgae Chlorella vulgaris to the composition of feed for trout fish, which allows to increase the growth rates and maintain the physiological state of fish at the appropriate level.
Добавил в систему: Маторин Дмитрий Николаевич

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STUDY OF THE INFLUENCE OF BIOLOGICALLY ACTIVE ADDITIVE USING MICROALGAE ON THE GROWTH AND MICROFLORA OF RAINBOW TROUTстатья

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