Control of the activity of an enzyme immobilized into thermoreversible hydrogel

Авторы: Sigolaeva L.V., Eremeev N.L., Kazanskaya N.F.
Сборник: Proceedings of SPIE (3rd International Conference on Intelligent Materials and 3rd European Conference on Smart Structures and Materials)
Том: 2779
Год издания: 1996
Место издания: SPIE--The International Society for Optical Engineering
Первая страница: 66
Последняя страница: 71
DOI: 10.1117/12.237023
Аннотация: The effect of phase transition of a thermosensitive matrix on temperature dependencies of observed activity, equilibrium, and kinetic constants of the enzymatic reaction was studied using (alpha) -chymotrypsin immobilized into thermosensitive poly-N-isopropylacrylamide gel as a model system. The behavior of the system is determined by the properties of the enzyme at temperatures less than critical temperature corresponding to phase transition of the polymeric gel but by the properties of the thermosensitive polymeric matrix at temperatures higher than that critical temperature. The phase transition of the thermosensitive gel was shown to affect weakly temperature dependencies of equilibrium parameters of enzymatic reactions. All reversible effects of the reduction of the enzymatic activity at high temperature are exclusively due to the changes in temperature behavior of kinetic parameters of the enzymatic reaction.
Добавил в систему: Сиголаева Лариса Викторовна

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