Heterologous Expression and Isolation of Influenza A Virus Nuclear Export Protein NEP

Golovko, A.O.; Koroleva, O.N.; Drutsa, V.L.

Информация о цитировании статьи получена из Web of Science, Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 15 апреля 2020 г.

Авторы: Golovko A.O., Koroleva O.N., Drutsa V.L.
Журнал: Biochemistry (Moscow)
Том: 82
Номер: 12
Год издания: 2017
Издательство: Pleiades Publishing, Ltd
Местоположение издательства: Road Town, United Kingdom
Первая страница: 1529
Последняя страница: 1537
DOI: 10.1134/S0006297917120124
Аннотация: Influenza A virus nuclear export protein NEP (NS2, 14.4 kDa) plays a key role in various steps of the virus life cycle. Highly purified protein preparations are required for structural and functional studies. In this study, we designed a series of Escherichia coli plasmid constructs for highly efficient expression of the NEP gene under control of the constitutive trp promoter. An efficient method for extraction of NEP from inclusion bodies based on dodecyl sulfate treatment was developed. Preparations of purified NEP with either N- or C-terminal (His)6-tag were obtained using Ni-NTA agarose affinity chromatography with yield of more than 20 mg per liter of culture. According to CD data, the secondary structure of the proteins matched that of natural NEP. A high propensity of NEP to aggregate over a wide range of conditions was observed.
Добавил в систему: Друца Валерий Львович

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Heterologous Expression and Isolation of Influenza A Virus Nuclear Export Protein NEPстатья

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