Evaluation of aliphatic/aromatic compounds and fluorophores in dissolved organic matter of contrasting natural waters by SEC-HPLC with multi-wavelength absorbance and fluorescence detections

Trubetskaya, O.E.; Richard, C.; Patsaeva, S.V.; Trubetskoj, O.A.

Авторы: Trubetskaya Olga E., Richard Claire, Patsaeva Svetlana V., Trubetskoj Oleg A.
Журнал: Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
Том: 238
Номер: 5 September 2020, 118450
Год издания: 2020
Издательство: Pergamon Press Ltd.
Местоположение издательства: United Kingdom
Первая страница: 118450
Последняя страница: 118450
DOI: 10.1016/j.saa.2020.118450
Аннотация: The analytical high performance size exclusion liquid chromatography (SEC-HPLC) with multi-wavelength absorbance and fluorescence detections and fluorescence 3D-excitation/emission matrix (EEM) were used for the analysis of average molecular size (MS), molecular size distribution of aliphatic/aromatic compounds and fluorophores in dissolved organic matter (DOM) from Suwannee River (SRDOM) and two Karelian lakes (Vodoprovodnoe Lake - L1DOM and Onego Lake - L2DOM). The average MS of DOM samples varied in the order SRDOM > L1DOM > L2DOM. The absorbance ratios A250/A365 and A210/A254 have been used for the DOM samples characterization. The absorbance ratio A250/A365 provided significant information about average MS of the bulk aquatic DOMs but was not exactly correlated with the results of SEC-HPLC data. The absorbance ratio A210/A254 in combination with SEC was well correlated with the content of DOM aliphatic/aromatic compounds and could be used as an important water quality index to estimate the level of protein-like material in the aquatic DOM. Regardless of the origin (river or lakes), different geographical locations and different average MS of DOM, several similar types of humic-like SEC-separated fluorophores were found within the samples. In all DOM samples the decrease of the fluorophores emission maxima paralleled the decrease of their relative MS. The combination of SEC-HPLC with multi-wavelength fluorescence and absorbance detection and EEM analyses appears very useful for DOM characterization and for tracking of microbial activity resulting from anthropogenic and/or eutrophic impact in aquatic environments.
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Evaluation of aliphatic/aromatic compounds and fluorophores in dissolved organic matter of contrasting natural waters by SEC-HPLC with multi-wavelength absorbance and fluorescence detectionsстатья Исследовательская статья

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