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Electron transport in Tradescantia leaves acclimated to high and low light: Thermoluminescence, PAM-fluorometry and EPR studies. Photosynthesis Researchстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 25 ноября 2020 г.
- Авторы: Kalmatskaya O.A., Trubitsin B.V., Suslichenko I.S., Karavaev V.A., Tikhonov A.N.
- Журнал: Photosynthesis Research
- Том: 146
- Год издания: 2020
- Издательство: Springer Nature
- Местоположение издательства: Switzerland
- Первая страница: 123
- Последняя страница: 141
- DOI: 10.1007/s11120-020-00767-2
- Аннотация: Using thermoluminescence, PAM-fuorometry, and electron paramagnetic resonance (EPR) for assaying electron transport processes in chloroplasts in situ, we have compared photosynthetic characteristics in Tradescantia fuminensis leaves grown under low light (LL, 50–125 µmol photons m−2 s−1) or high light (HL, 875–1000 µmol photons m−2 s−1) condition. We found diferences in the thermoluminescence (TL) spectra of LL- and HL-acclimated leaves. The LL and HL leaves show diferent proportions of the Q (~ 0 °C) and B (~ 25–30 °C) bands in their TL spectra; the ratios of the “light sums” of the Q and B bands being SQ/SB ≈ 1/1 (LL) and SQ/SB ≈ 1/3 (HL). This suggests the existence of diferent redox states of electron carriers on the acceptor side of PSII in LL and HL leaves, which may be afected, in particular, by diferent capacities of their photo-reducible PQ pools. Enhanced content of PQ in chloroplasts of LL leaves may be the reason for an efcient performance of photosynthesis at low irradiance. Kinetic studies of slow induction of Chl a fuorescence and measurements of P700 photooxidation by EPR demonstrate that HL leaves have faster (about 2 times) response to switching on actinic light as compared to LL leaves grown at moderate irradiation. HL leaves also show higher non-photochemical quenching (NPQ) of Chl a fuorescence. These properties of HL leaves (faster response to light and generation of enhanced NPQ) refect the fexibility of their photosynthetic apparatus, providing sustainability and rapid response to fuctuations of environmental light intensity and solar stress resistance. Analysis of time-courses of the EPR signals of P+ 700 induced by far-red (λmax = 707 nm), exciting predominantly PSI, and white light, exciting both PSI and PSII, suggests that there is a contribution of cyclic electron fow around PSI to electron fow through PSI in HL leaves. The data obtained are discussed in terms of photosynthetic apparatus sustainability of HL and LL leaves under variable irradiation conditions.
- Добавил в систему: Тихонов Александр Николаевич