Development of a latex particles-based lateral flow immunoassay for group determination of macrolide antibiotics in breast milkстатья
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Дата последнего поиска статьи во внешних источниках: 24 сентября 2020 г.
Аннотация:A lateral flow immunoassay (LFIA) using latex-labeled group-specific polyclonal antibodies was developed for the rapid simultaneous detection of six macrolide antibiotics in the breast milk. The results of LFIA test could be interpreted visually and instrumentally. The visual limits of detection (vLOD) were 1, 50, 1, 10, 10, and 1000 ng/mL for roxithromycin, azithromycin, clarithromycin, erythromycin, dirithromycin, and oleandomycin in buffer, respectively. The calculated limits of detection (cLOD) were respectively 0.12, 2.1, 0.15, 1.4, 2.4, and 3.3 ng/mL. To avoid a strong influence of matrix-mediated breast milk of a very diverse and variable composition, a sample pretreatment was proposed. The six macrolides could be visually detectable in breast milk after 20-min pretreatment at their content of 10- 1000 ng/mL. The recovery rate from the spiked samples carried out using strip scanner device ranged from 71 % to 110%, and accuracy of the results was from 3% to 14% (RSD). The described rapid on-site diagnostic assay format can be useful for monitoring the content of antibiotics in breast milk during macrolide treatment to ensure safe breastfeeding of infants.