Prussian blue nanoparticles with peroxidase-mimicking properties in a dual immunoassays for glycocholic acid

He, Q.; Huiyi, Y.; Yingshan, C.; Ding, S.; Xiping, C.; Chunguo, Z.; Huanxin, X.; A, E.S.; Yanxiong, F.; Suqing, Z.

Информация о цитировании статьи получена из Web of Science, Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 24 сентября 2020 г.

Авторы: Qiyi He, Yang Huiyi, Chen Yingshan, Shen Ding, Cui Xiping, Zhang Chunguo, Xiao Huanxin, Eremin Sergei A., Fang Yanxiong, Zhao Suqing
Журнал: Journal of Pharmaceutical and Biomedical Analysis
Том: 187
Год издания: 2020
Издательство: Elsevier BV
Местоположение издательства: Netherlands
Номер статьи: 113317
DOI: 10.1016/j.jpba.2020.113317
Аннотация: Prussian Blue nanoparticles (PBNPs) were utilized in a lateral flow immunoassay (LFA) and in an indirect competitive nanozyme-linked immunosorbent assay (icELISA), respectively, for their intense blue color and peroxidase (POx) -like activity. The PBNPs with good POx-like activity was linked to the antibody. Under the optimal parameters, both the PBNP-icELISA and PBNP-LFA perform very well. The icELISA has an IC50 value of 190?ng/mL, the working range extends from 29 to 1200?ng/mL, and the limit of detection is 22?ng/mL. The visual cut-off limit is 10?ng/mL. The dual immunoassay was used to quantify glycocholic acid in spiked human urine. Excellent recoveries and correlation between the two methods were observed.
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Prussian blue nanoparticles with peroxidase-mimicking properties in a dual immunoassays for glycocholic acidстатья

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