|
ИСТИНА |
Войти в систему Регистрация |
ИСТИНА ИНХС РАН |
||
The Arabidopsis thaliana AtPrx53 peroxidase gene naturally occurring dimorphism: association with developmental traits and allozyme mobilityтезисы доклада
- Автор: Kupriyanova E.V.
- Сборник: FESPB conference
- Тезисы
- Год издания: 2008
- Место издания: Tampere, Finland
- Аннотация: The sequenced genomes of Arabidopsis thaliana proved to contain 73 peroxidase genes, however, the data on the functions of particular peroxidase genes and on the level of their variation remain scarce. The DNA polymorphism in the AtPrx53 gene was analyzed in 20 A.thaliana accessions. This gene encodes at least 3 anionic peroxidase isoforms, their total activity in the leaves, stem, and pods amounts to over 45, 60, and 80% of the activity of all anionic peroxidases. The AtPrx53 mRNA expression level is influenced by light intensity, high and low temperature, drought, paraquat and exogenic auxin and gibberelin. As in some other loci studied in A. thaliana, two divergent sequence types were detected in the AtPrx53 gene (Col and Dj-like haplotypes). Two of the amino acid substitutions (Phe/Ser180and Asp/Asn270) could be responsible for the difference in electrophoretic mobility of AtPrx53 allozymes. The haplotype Col characterized by faster mobility of three isoforms in comparison with the Dj haplotype. There is a significant association between the haplotype and several developmental traits: leaf number, flowering time, main stem height etc. These data indicate functional importance of two substitutions in AtPrx53 peroxidase and great significance of AtPrx53 gene for plant development. The study was supported by grants from RFBR program (07-04-12077-ofr) and RAS Programs "Gene Pool Dynamics" and "Origin and Evolution of Biosphere".
- Добавил в систему: Куприянова Евгения Владимировна