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Possible Contribution of mitotic gene conversion in the conservation of maternal haplotype of peroxidase genesтезисы доклада
- Авторы: Kupriyanova E., Ezhova T.A.
- Сборник: XVIII Congress of the Federation of European Societies of Plant Biology (FESPB)
- Тезисы
- Год издания: 2010
- Место издания: Valencia, Spain
- Первая страница: 133
- Последняя страница: 133
- Аннотация: The distribution of variation in a genome is the result of an intricate interplay between mutation, recombination, selection, and demography and is influenced by the reproductive system and ecological constraints. Important observations have emerged from the analyses of several Arabidopsis loci that have been subjected to comparative sequencing in this cruciferous weed: (i) a number of genes have alleles that fall into two distinct classes of haplotypes, and (ii) there is more recombination than might be expected, given that Arabidopsis is a selfer. Molecular-genetic mapping of the area of localization of tandemly duplicated anionic peroxidase genes AtPrx53 and AtPrx54 was performed. It was stated that this area is the recombination hotspot where recombination frequency was 375 times higher than the V chromosome average. The cloning and DNA sequencing of recombinant chromosomes has shown that the alleles of peroxidase genes posess mosaic structure. This indicates that abnormal high frequency of recombination was the result of conversion mechanisms. The analysis of codominant DNA marker’s segregation demonstrated that conversion events lead to the elimination of heterozygosity in hybrid plants; a proportion of plants homozygous for the maternal alleles of AtPrx53 and AtPrx54 was increased. Thus, unlike other recombination hotspot, which generates polymorphism by creating new recombinant alleles, we identified conversion hotspot, which eliminate heterozygosity and restore the maternal haplotype. It can be suggest that mitotic gene conversion can be used by plants to preserve of maternal haplotypes which possess higher adaptive value in specific environmental conditions. The study was supported by grants from RFBR program ( 09-04-01639; 09–04–12216).
- Добавил в систему: Куприянова Евгения Владимировна