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Metabolic profiling of aromatic compounds in cerebrospinal fluid of neurosurgical patients using microextraction by packed sorbent and liquid‐liquid extraction with the following gas chromatography‐mass spectrometry analysisстатья
Информация о цитировании статьи получена из
Web of Science
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 4 марта 2022 г.
- Авторы: Pautova Alisa K., Khesina Zoya B., Litvinova Tatiana N., Revelsky Alexander I., Beloborodova Natalia V.
- Журнал: Biomedical Chromatography
- Том: 35
- Номер: 2
- Год издания: 2021
- Издательство: John Wiley & Sons Inc.
- Местоположение издательства: United States
- Первая страница: e4969
- DOI: 10.1002/bmc.4969
- Аннотация: A new approach to the quantitative analysis of aromatic metabolites in cerebrospinal fluid samples of neurosurgical patients based on microextraction by packed sorbent coupled with derivatization and GC‐MS was developed. The analytical characteristics such as recoveries (40‐90%), LOD (0.1‐0.3 μM) and LOQ (0.4‐0.7 μM) values, accuracy less than ±20% and precision less than 20%, linear correlations (R2 ≥ 0.99) over 0.4‐10 μM range of concentrations demonstrated that microextraction by packed sorbent provides the results for the quantitative analysis of target compounds comparable with those for liquid‐liquid extraction. Similar results were achieved using 40 μL of sample for microextraction by packed sorbent instead of 200 μL for liquid‐liquid extraction.Benzoic, 3‐phenylpropionic, 3‐phenyllactic, 4‐hydroxybenzoic, 2‐(4‐hydroxyphenyl)acetic, homovanillic, and 3‐(4‐hydroxyphenyl)lactic acids were found in cerebrospinal fluid samples (n=138) of neurosurgical patients in lower concentrations than in serum samples (n=110) of critically ill patients. Analysis of the cerebrospinal fluid and serum samples taken at the same time from neurosurgical patients (n=5) revealed the similar results for patients without infection and multidirectional results for patients with the central nervous system infection. Our preliminary results demonstrate the necessity for the further evaluating the aromatic compound profile in cerebrospinal fluid for its subsequent verification as potential diagnostic markers
- Добавил в систему: Ревельский Александр Игоревич