Spectroscopic investigations of fluorescence behaviour, role and function of the long-wavelength pigments of Photosystem Iстатья
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Дата последнего поиска статьи во внешних источниках: 31 декабря 2013 г.
Аннотация:A method was established for quantitative evaluation of fluorescence spectra of intact leaves without expensive sample preparation. Fluorescence induction and excitation effects were used to evaluate the contribution of Photosystem I to the long-wavelength fluorescence of intact leaves at room temperature which appeared to be about 25%. To characterize its spectral line-shape we studied low-temperature fluorescence. The connection of thesethree methods made it possible to determine the quantitative spectra of both photosystems in intact leaves. Investigations of fluorescence decay kinetics on isolated Photosystem I subchloroplast particles provided information about functional aspects of the long-wavelength pericentral antenna pigments. It appeared that these pericentral pigments are not essential for transferring excitation energy toward photochemically active reaction centres. Only if the reaction centres were closed the pericentral antenna would act as a protection guard against photodestruction.