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Immunohistochemical detection of the activation of CREB and c-Fos transcription factors in the nervous system of the terrestrial snail induced by pentylenetetrazoleстатья
Информация о цитировании статьи получена из
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 4 февраля 2014 г.
- Авторы: Efimova O.I., Ierusalimskii V.N., Anokhin K.V., Balaban P.M.
- Журнал: Neuroscience and Behavioral Physiology
- Том: 37
- Номер: 9
- Год издания: 2007
- Издательство: Kluwer Academic/Plenum Publishers
- Местоположение издательства: United States
- Первая страница: 853
- Последняя страница: 856
- DOI: 10.1007/s11055-007-0092-6
- Аннотация: Phosphorylation of CREB transcription factor and expression of the immediate early gene c-fos in the brains of vertebrates play a key role in the molecular genetic mechanisms of long-term neuronal plasticity. The present study identifies the conditions for immunohistochemical detection of pCREB and c-Fos in the nervous system of the mollusk Helix lucorum (Pulmonata: Stylommatophora); activation of these transcription factors was demonstrated after administration of the convulsive agent pentylenetetrazole. Basal pCREB and c-Fos levels in the central nervous system of intact animals were low. Injection of pentylenetetrazole at a dose of 600 mg/kg evoked characteristic stereotypical motor responses, along with sharp reductions in the phosphorylation of CREB1 and expression of the immediate early gene c-fos, this also occurring in identified neurons. Double immunofluorescent labeling of pCREB and c-Fos showed that expression of c-Fos transcription factor was seen only in pCREB-immunoreactive neurons. These data provide evidence that activation of pCREB and c-Fos transcription factors can be used as molecular markers for mapping the processes of neuronal plasticity in the nervous systems of mollusks.
- Добавил в систему: Балабан Павел Милославович