The cytoplasmic tail of Influenza A/H1N1 virus hemagglutinin is β-structural

Khrustalev, V.V.; Kordyukova, L.V.; Arutyunyan, A.M.; Poboinev, V.V.; Khrustaleva, T.A.; Stojarov, A.N.; Baratova, L.A.; Sapon, A.S.; Lugin, V.G.

Авторы: Khrustalev V.V., Kordyukova L.V., Arutyunyan A.M., Poboinev V.V., Khrustaleva T.A., Stojarov A.N., Baratova L.A., Sapon A.S., Lugin V.G.
Журнал: Journal of Biomolecular Structure and Dynamics
Том: 40
Номер: 10
Год издания: 2022
Издательство: Adenine Press
Местоположение издательства: United States
Первая страница: 4642
Последняя страница: 4661
DOI: 10.1080/07391102.2020.1860827
Аннотация: Influenza A/H1N1 virus hemagglutinin (HA) is an integral type I glycoprotein that contains a large glycosylated ectodomain, a transmembrane domain, and a cytoplasmic tail (CT) of 10-14 amino acid residues. There are absolutely no data on the secondary or tertiary structure of the HA CT, which is important for virus pathogenesis. Three highly conserved cysteines are post-translationally modified by the attachment of fatty acid residues that pin the CT to the lipid membrane inside the virion. We applied circular dichroism (CD) and fluorescence spectroscopy analysis to examine four synthetic peptides corresponding to 14-15 C-terminal residues of H1 subtype HA (NH2-WMCSNGSLQCRICI-COOH; NH2-FWMCSNGSLQCRICI-COOH), with free or acetaminomethylated cysteines, in the reduced or non-reduced state, at various pH values and temperatures. The CD analysis detected the formation of a β-structure (30–65% according to the new BeStSel algorithm), in addition to an unstructured random coil, in every peptide in various conditions. It was completely or partially recognized as an antiparallel β-structure that was also confirmed by the multi-bounce Horizontal Attenuated Total Reflectance Fourier Transformed Infrared (HATR-FTIR) spectroscopy analysis. According to the experimental data, as well as 3D modeling, we assume that the amino acid sequence corresponding to the HA CT may form a short antiparallel β-structure under the lipid membrane within a virion.
Добавил в систему: Кордюкова Лариса Валентиновна

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The cytoplasmic tail of Influenza A/H1N1 virus hemagglutinin is β-structuralстатья

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