In Vitro and In Vivo Studies of Biodegradability and Biocompatibility of Poly(εCL)-b-Poly(EtOEP)-Based Films

Nifant’ev, I.; Shlyakhtin, A.; Komarov, P.; Tavtorkin, A.; Kananykhina, E.; Elchaninov, A.; Vishnyakova, P.; Fatkhudinov, T.; Ivchenko, P.

Авторы: Nifant’ev Ilya, Shlyakhtin Andrey, Komarov Pavel, Tavtorkin Alexander, Kananykhina Evgeniya, Elchaninov Andrey, Vishnyakova Polina, Fatkhudinov Timur, Ivchenko Pavel
Журнал: Polymers
Том: 12
Номер: 12
Год издания: 2020
Издательство: MDPI
Местоположение издательства: Basel, Switzerland
Номер статьи: 3039
DOI: 10.3390/polym12123039
Аннотация: The control of surface bioadhesive properties of the subcutaneous implants is essentialfor the development of biosensors and controlled drug release devices.Poly(alkyl ethylenephosphate)-based (co)polymers are structurally versatile, biocompatible and biodegradable, and maybe regarded as an alternative to poly(ethylene glycol) (PEG) copolymers in the creation of antiadhesivematerials. The present work reports the synthesis of block copolymers ofε-caprolactone (εCL) and2-ethoxy-1,3,2-dioxaphospholane-2-oxide (ethyl ethylene phosphate, EtOEP) with different content ofEtOEP fragments, preparation of polymer films, and the results of the study of the impact of EtOEP/εCLratio on the hydrophilicity (contact angle of wetting), hydrolytic stability, cytotoxicity, protein andcell adhesion, and cell proliferation using umbilical cord multipotent stem cells. It was found that theincrease of EtOEP/εCL ratio results in increase of hydrophilicity of the polymer films with loweringof the protein and cell adhesion. MTT cytotoxicity test showed no significant deviations in toxicity ofpoly(εCL) and poly(εCL)-b-poly(EtOEP)-based films. The influence of the length of poly(EtOEP)chainin block-copolymers on fibrotic reactions was analyzed using subcutaneous implantation experiments(Wistar line rats), the increase of the width of the fibrous capsule correlated with higher EtOEP/εCLratio. However, the copolymer-based film with highest content of polyphosphate had been subjectedto faster degradation with a formation of developed contact surface of poly(εCL). The rate of thedegradation of polyphosphatein vivowas significantly higher than the rate of the degradationof polyphosphatein vitro, which only confirms an objective value ofin vivoexperiments in thedevelopment of polymer materials for biomedical applications
Добавил в систему: Ивченко Павел Васильевич

	ИСТИНА	Войти в систему Регистрация
	ИСТИНА ИНХС РАН
	Главная Поиск Статистика О проекте Помощь

ИСТИНА

ИСТИНА ИНХС РАН

In Vitro and In Vivo Studies of Biodegradability and Biocompatibility of Poly(εCL)-b-Poly(EtOEP)-Based Filmsстатья

Прикрепленные файлы