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Stimulation by cytochrome c of the external pathway of NADH oxidation and ascorbate oxidation in the presence of TMPDстатья
Информация о цитировании статьи получена из
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 29 ноября 2013 г.
- Авторы: Brailovskaya I.V., Korotkov S.M., Emel'yanova L.V., Mokhova E.N.
- Журнал: Doklady Biochemistry and Biophysics
- Том: 408
- Год издания: 2006
- Издательство: M A I K Nauka - Interperiodica
- Местоположение издательства: Russian Federation
- Первая страница: 123
- Последняя страница: 126
- DOI: 10.1134/S1607672906030045
- Аннотация: A significantly increased interest of researchers to the mechanisms of cytochrome c release form mitochondria is determined by the role of this process in the induction of apoptosis. It was believed for a long time that cytochrome c can enter mitochondria and be released from them only after the outer membrane damage [1]. A considerable part of cytochrome c is released from mitochondria after their hypotonic treatment, which disrupts the integrity of their outer membrane, and subsequent incubation in media containing high concentrations of cations, which enables desorption of cytochrome c from the outer surface of the inner mitochondrial membrane [2]. Desorption of cytochrome c also increases as a result of peroxidation of cardiolipin in the inner membrane, with negative groups of which cytochrome c is bound in native mitochondria [3].However, under certain conditions, cytochrome c may be released from mitochondria in the absence of damages in the outer membrane, through a cytochrome c-permeable channel formed in it [4, 5]. On the basis of these and other data, we assumed that a pronounced stimulation of mitochondrial respiration by cytochrome c does not necessarily indicate the disruption of integrity of their outer membrane, which calls into question the relevance of estimation of the proportion of mitochondria with a damaged outer membrane by the stimulation of their respiration by exogenous cytochrome c.
- Добавил в систему: Мохова Елена Николаевна