Time-Resolved Electrometric Study of the F→O Transition in Cytochrome c Oxidase. The Effect of Zn2+ Ions on the Positive Side of the Membraneстатья
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Дата последнего поиска статьи во внешних источниках: 9 апреля 2021 г.
Аннотация:The effect of Zn2+ on the P-side of proteoliposomes containing membraneincorporated Rhodobacter sphaeroides cytochrome c oxidase was investigated by the time-resolved electrometrics following a single electron injection into the enzyme prepared in the F state. The wild type enzyme was examined along with the two mutants, N139D and D132N. All obtained data indicate that the primary effect of Zn2+ added from the Pside of the membrane is slowing of the pumped pro ton release from the proton loading site (PLS) to the bulk aqueous phase on the Pside of the membrane. The results strongly suggest the presence of two pathways by which the pumped proton can exit the protein from the PLS and of two separate binding sites for Zn2+. A model is presented to explain the influence of Zn2+ on the kinetics of membrane potential generation by the wild-type COX, as well as by the N139D and D132N mutants