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HETEROLOGOUS EXPRESSION OF FUNCTIONALLY ACTIVE HUMAN CYTOCHROMES P450 - CYTOCHROME P450IIIA4 CATALYZES CONVERSION OF THE ANABOLIC-STEROID HORMONE METHANDROSTENOLONEстатья
Информация о цитировании статьи получена из
Web of Science
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 27 мая 2015 г.
- Авторы: KRYNETSKII EY, KOVALEVA IE, LUZIKOV VN
- Журнал: Biochemistry (Moscow)
- Том: 59
- Номер: 2
- Год издания: 1994
- Издательство: Pleiades Publishing, Ltd
- Местоположение издательства: Road Town, United Kingdom
- Первая страница: 203
- Последняя страница: 206
- Аннотация: Cytochrome P450IIIA4 has been expression in the yeast Saccharomyces cerevisiae using the pYeDP 1-8/2 shuttle-vector. The microsomal fraction isolated from the transformed yeast was used for conversion of methandrostenolone (MA), an anabolic steroid hormone. The reaction products of the microsomal oxidation were analyzed by HPLC and two-dimensional TLC. Microsomes of the yeast expressing human cytochrome P450IIIA4 catalyze conversion of MA to its 6 beta-hydroxy derivative. A similar product is formed during the reaction catalyzed by human liver microsomes. Thus, the use of the heterologous system expressing human liver cytochrome P450IIIA4 established its involvement in the MA metabolism. This system simulates the first phase of conversion of the drug in liver cells.
- Добавил в систему: Ковалева Ирина Евгеньевна