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Fluorescence Polarization-Based Bioassays: New Horizonsстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 30 апреля 2021 г.
- Авторы: Hendrickson Olga D., Taranova Nadezhda A., Zherdev Anatoly V., Dzantiev Boris B., Eremin Sergei A.
- Журнал: Sensors
- Том: 20
- Номер: 24
- Год издания: 2020
- Издательство: MDPI
- Местоположение издательства: Basel, Switzerland
- Первая страница: 7132
- Последняя страница: 7132
- Номер статьи: 7132
- DOI: 10.3390/s20247132
- Аннотация: Fluorescence polarization holds considerable promise for bioanalytical systems because it allows the detection of selective interactions in real time and a choice of fluorophores, the detection of which the biosample matrix does not influence; thus, their choice simplifies and accelerates the preparation of samples. For decades, these possibilities were successfully applied in fluorescence polarization immunoassays based on di_erences in the polarization of fluorophore emissions excited by plane-polarized light, whether in a free state or as part of an immune complex. However, the results of recent studies demonstrate the e_cacy of fluorescence polarization as a detected signal in many bioanalytical methods. This review summarizes and comparatively characterizes these developments. It considers the integration of fluorescence polarization with the use of alternative receptor molecules and various fluorophores; di_erent schemes for the formation of detectable complexes and the amplification of the signals generated by them. New techniques for the detection of metal ions, nucleic acids, and enzymatic reactions based on fluorescence polarization are also considered
- Добавил в систему: Еремин Сергей Александрович
Прикрепленные файлы
| № | Имя | Описание | Имя файла | Размер | Добавлен |
|---|---|---|---|---|---|
| 1. | 20_Sensors20_7132_FP_bioassay.pdf | 20_Sensors20_7132_FP_bioassay.pdf | 2,5 МБ | 15 марта 2021 [Sergei_A_EREMIN] |