Местоположение издательства:Wageningen, The Netherlands
Первая страница:195
Последняя страница:202
Аннотация:Stacking (pyramiding) several late blight (LB) resistance genes of diverse race specificity in one and the same potato plant provides for high, broad spectrum and durable resistance. Forty clones of standard potato varieties and interspecific hybrids comprising genetic material from two to seven wild Solanum species were evaluated for LB resistance and screened with SCAR markers for resistance genes R1, R2/Rpi-blb3, R3a, R3b and RB/Rpi-blb1. LB resistance of the hybrids was significantly related to the presence of the markers for resistance genes. However, the correlation was weaker than expected: apparently a considerable portion of LB resistance depended on the genes insufficiently researched in wild progenitors of interspecific hybrids. To verify the markers, we compared their sequences to those of the prototype genes from S. demissum and S. stoloniferum. In one case of incomplete match, indels and stop codons in the marker sequences can explain low LB resistance of the hybrid. In other cases, the observed polymorphisms in the marker sequences presume that the gene in question was probably introgressed from Solanum species other than S. demissum and S. stoloniferum.