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SEARCHING AMONG WILD SOLANUM SPECIES FOR HOMOLOGUES OF RB/RPI-BLB1 GENE CONFERRING DURABLE LATE BLIGHT RESISTANCEстатья
- Авторы: PANKIN A.A., SOKOLOVA E.A., ROGOZINA E.V., KUZNETSOVA M.A., DEAHL K.L., JONES R.W., KHAVKIN E.E.
- Журнал: PPO-SPECIAL REPORT
- Номер: 14
- Год издания: 2010
- Издательство: Applied Plant Research
- Местоположение издательства: Wageningen, The Netherlands
- Первая страница: 277
- Последняя страница: 284
- Аннотация: Solanum bulbocastanum comprising a CC-NBS-LRR gene RB/Rpi-blb1 confers broad-spectrum resistance to Phytophthora infestans and is currently employed in potato breeding for durable late blight (LB) resistance. Genomes of several Solanum species were reported to contain RB homologues with confirmed broad-spectrum defence function. With the discovery that novel P. infestans races break LB resistance mediated by the genes of broad-spectrum specificity, pyramiding several RB-like genes from various Solanum species in a single potato cultivar seems a promising approach to durable LB resistance. Here we report early evidence on RB-like sequences in the wide range of Solanum species section Petota. The panel of Solanum species was screened with three RB-related PCR markers. RB-like sequences were found in every tested Solanum accession suggesting universal distribution of RB structural homologues among Solanum genomes, while the marker RB-629 corresponding to the RB gene was found in 14 species. The phylogenetic analysis of RB-629 sequences suggested highly conserved pattern of polymorphisms that was neither species- nor seriesspecific. Apparently, duplication and evolution of RB-like loci preceded Solanum speciation. Marker presence and particular haplotypes were not immediately associated with high LB resistance. http://130.226.173.223/euroblight/Workshop/2010Arras/Proceedings/Page277_284_Pankin_web.pdf
- Добавил в систему: Кузнецова Мария Алексеевна