Molecular model of a sensor of two-component signaling system

Ryzhykau, Y.L.; Orekhov, P.S.; Rulev, M.I.; Vlasov, A.V.; Melnikov, I.A.; Volkov, D.A.; Nikolaev, M.Y.; Zabelskii, D.V.; Murugova, T.N.; Chupin, V.V.; Rogachev, A.V.; Gruzinov, A.Y.; Svergun, D.I.; Brennich, M.E.; Gushchin, I.Y.; Soler-Lopez, M.; Bothe, A.; Büldt, G.; Leonard, G.; Engelhard, M.; Kuklin, A.I.; Gordeliy, V.I.

Статья опубликована в высокорейтинговом журнале

Информация о цитировании статьи получена из Web of Science, Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 7 июля 2021 г.

Авторы: Ryzhykau Yury L., Orekhov Philipp S., Rulev Maksim I., Vlasov Alexey V., Melnikov Igor A., Volkov Dmytro A., Nikolaev Mikhail Yu, Zabelskii Dmitrii V., Murugova Tatiana N., Chupin Vladimir V., Rogachev Andrey V., Gruzinov Andrey Yu, Svergun Dmitri I., Brennich Martha E., Gushchin Ivan Yu, Soler-Lopez Montserrat, Bothe Arne, Büldt Georg, Leonard Gordon, Engelhard Martin, Kuklin Alexander I., Gordeliy Valentin I.
Журнал: Scientific reports
Том: 11
Номер: 1
Год издания: 2021
Издательство: Nature Publishing Group
Местоположение издательства: United Kingdom
Номер статьи: 10774
DOI: 10.1038/s41598-021-89613-6
Аннотация: Two-component systems (TCS) are widespread signaling systems present in all domains of life. TCS typically consist of a signal receptor/transducer and a response regulator. The receptors (histidine kinases, chemoreceptors and photoreceptors) are often embedded in the membrane and have a similar modular structure. Chemoreceptors were shown to function in highly ordered arrays, with trimers of dimers being the smallest functional unit. However, much less is known about photoreceptors. Here, we use small-angle scattering (SAS) to show that detergent-solubilized sensory rhodopsin II in complex with its cognate transducer forms dimers at low salt concentration, which associate into trimers of dimers at higher buffer molarities. We then fit an atomistic model of the whole complex into the SAS data. The obtained results suggest that the trimer of dimers is "tripod"-shaped and that the contacts between the dimers occur only through their cytoplasmic regions, whereas the transmembrane regions remain unconnected.
Добавил в систему: Орехов Филипп Сергеевич

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Molecular model of a sensor of two-component signaling systemстатья

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