Methanol emission by wounded plant enhances mice attraction: behavioral and gene-expression analysisстатья
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Дата последнего поиска статьи во внешних источниках: 29 мая 2015 г.
Аннотация:Pectin methylesterase (PME, EC: 3.1.1.11) is a ubiquitous multifunctional enzymatic component of the plant cell wall which plays role in defense against invading pathogens and also participates in cell wall modulation during general plant growth. PME-mediated pectin demethylation results in production of methanol. For a long time gaseous methanol was considered as a by-product of the PME-mediated pectin demethylation. However, our recent data has shown the role of methanol in plant-to-plant communication. Plant leaf wounding resulted in burst of methanol production and emission accompanied by up-regulating mostly defense and cell-to-cell communication genes of intact neighboring plant.
Here, we examined the role of methanol in plant-animal interaction. First, we showed that PME-containing citrus pectin [pectin(PME+)] transiently increased methanol content in mice plasma. Using animal cell culture, we selected and isolated the following methanol-induced genes (MIGs) for further analysis: glyceraldehyde 3-phosphate dehydrogenase, Tax1 (T-cell leukemia virus type I) binding protein 1, sorting nexin family member 27 and cyclin A2. The quantitative real-time PCR determination of mRNA validated changes in MIGs expression in the brain of mice 2 hours after methanol or pectin(PME+) ingestion. To show the role of methanol in mice attraction we exploited the Y-maze approach and two-choice test. The clear mice preferences of methanol or volatiles from damaged Nicotiana benthamiana and Brassica rapa subsp. pekinensis over those undamaged plants were shown. It's important to emphasize that inhalation of vapors of wounded leaves was accompanied by growth of methanol content in mice blood. Moreover, the quantitative real-time PCR determination of mRNA confirmed changes in MIGs expression in the brain of mice after inhalation of methanol or vapors of wounded leaves. We concluded that methanol may be involved in plant-animal signaling.