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Application of Polymer Microspheres for Assessing the Roleof Mechanical Factors in the Formationof Neutrophilic Extracellular Trapsстатья
Дата последнего поиска статьи во внешних источниках: 18 августа 2021 г.
- Авторы: Basyreva L.Yu, Fedorova E.A., Polonskiy V.A., Skopintsev I.V., Luss A.L., Sivaev A.A., Gusev S.A., Shtilman M.I., Gritskova I.A.
- Журнал: Nanotechnologies in Russia
- Том: 16
- Номер: 1
- Год издания: 2021
- Издательство: Pleiades Publishing, Ltd
- Местоположение издательства: Road Town, United Kingdom
- Первая страница: 96
- Последняя страница: 102
- DOI: 10.1134/s263516762101002x
- Аннотация: At present, quite a large number of inducers forming neutrophilic extracellular traps (NET) have been described. Mechanical factors (MF) are of particular importance, since they are constantly acting as neutrophils pass through the microcirculatory system. An adequate choice of therapy for various diseases accompanied by excessive NET formation requires assessment of the ability of a patient’s neutrophils to form NET in response to various inducers. Studying the role of MF in neutrophil activation in patients presents certain difficulties associated with introducing expensive and complex recording systems into laboratory practice. A possible alternative to the existing approaches can be joint incubation of whole blood with hemocompatible polymer microspheres (PMS). To develop this approach, polystyrene microspheres containing quaternary ammonium groups were obtained, on the surface of which hemocompatible aggregates consisting of thiooctadecyl poly-N-vinyl-2-pyrrolidone (PMSnano) were attached. The data obtained indicate that with incubation of blood samples with PMSnano under gentle stirring, the amount of formed NET is statistically significantly higher than in a stationary incubation mode. This confirms the idea that MF are capable of activating neutrophils and triggering the NET formation process. The proposed approach to assessing the effect of MF on neutrophil activation processes owing to its ease of implementation may find wide application both in biomedical research and clinical laboratories.
- Добавил в систему: Лусс Анна Леонидовна