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Façade detergents as bicelle rim-forming agents for solution NMR spectroscopyстатья
- Авторы: Mineev K.S., Nadezhdin K.D., Goncharuk S.A., Arseniev A.S.
- Журнал: Nanotechnology Reviews
- Год издания: 2016
- DOI: 10.1515/ntrev-2016-0069
- Аннотация: Out of all membrane mimetics available for solution NMR spectroscopy, phospholipid bicelles are at most prospective. Unlike lipid-protein nanodiscs their size can be easily controlled in a wide range, and the exchange of matter between the particles can take place. However, recent studies revealed several major drawbacks of conventional DMPC/DHPC and DMPC/CHAPS bicelles. First, size of such bicelles can increase dramatically upon heating, and, second, rim-forming detergents of bicelles can cause the improper folding of the watersoluble globular domains of membrane proteins. In order to avoid these effects, we tested the Façade detergents as possible alternative rim-forming agents for small isotropic bicelles. In the present work we characterized the size of bicelles formed by Façade-EM and Façade-EPC as a function of temperature and lipid/detergent ratio by 1H NMR diffusion spectroscopy. Additionally, the denaturing effects of these two rim-forming agents were investigated using the junction of the transmembrane and intracellular domains of the p75 neurotrophin receptor (p75NTR) as a model object. We show that the use of Façades allows decreasing the temperature-dependent growth of bicelles. The ability of Façade-EM-based bicelles to support the native structure and soluble state of the p75NTR intracellular domain was also revealed.
- Добавил в систему: Гончарук Сергей Александрович