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Fluorescence polarization immunoassay for rapid determination of dehydroepiandrosterone in human urineстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 15 июля 2021 г.
- Авторы: Yang Huiyi, He Qiyi, Eremin Sergei A., Pan Junkang, Zou Yikui, Cui Xiping, Zhao Suqing
- Журнал: Analytical and Bioanalytical Chemistry
- Том: 413
- Номер: 17
- Год издания: 2021
- Издательство: Springer Verlag
- Местоположение издательства: Germany
- Первая страница: 4459
- Последняя страница: 4469
- DOI: 10.1007/s00216-021-03403-7
- Аннотация: In this paper, five fluorescein-labeled dehydroepiandrosterone (DHEA) derivatives (tracers) with different chain lengths between the fluorescein and hapten were synthesized and featured so as to establish a fluorescence polarization immunoassay (FPIA) for DHEA detection in human urine samples with previously prepared polyclonal antibody against DHEA. The outcomes of the structure of tracer on FPIA sensitivity were investigated. Under the optimal condition, the fluorescence polarization value (FP) decreases linearly in DHEA concentration, ranging from 1.6 to 243.3 ng mL−1, with the limit of detection of 1.1 ng mL−1 and IC50 value of 25.1 ng mL−1. Moreover, the developed FPIA was time-saving as it could complete the detection within 3 min. FPIA and commercial enzyme-linked immunosorbent assay kit were both applied to analyze the spiked human urine samples with DHEA. Excellent recoveries (92.1–108.0%) and satisfactory correlation coefficient (R2 = 0.98) were acquired with the two methods, indicating that the developed FPIA was a fast and efficient screening immunoassay with accuracy and sensitivity for DHEA detection in human urine samples.
- Добавил в систему: Еремин Сергей Александрович
Прикрепленные файлы
| № | Имя | Описание | Имя файла | Размер | Добавлен |
|---|---|---|---|---|---|
| 1. | abc413_4459.pdf | abc413_4459.pdf | 1,2 МБ | 13 июля 2021 [Sergei_A_EREMIN] |