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Partially Disordered Structure in Intravirus Coat Protein of Potyvirus Potato Virus Aстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 25 сентября 2013 г.
- Авторы: Ksenofontov AL, Paalme V., Arutyunyan AM, Semenyuk PI, Fedorova NV, Rumvolt R., Baratova LA, Jarvekulg L., Dobrov EN
- Журнал: PLoS ONE
- Том: 8
- Номер: 7
- Год издания: 2013
- Издательство: Public Library of Science
- Местоположение издательства: United States
- Первая страница: e67830
- DOI: 10.1371/journal.pone.0067830
- Аннотация: Potyviruses represent the most biologically successful group of plant viruses, but to our knowledge, this work is the first detailed study of physicochemical characteristics of potyvirus virions. We measured the UV absorption, far and near UV circular dichroism spectra, intrinsic fluorescence spectra, and differential scanning calorimetry (DSC) melting curves of intact particles of a potato virus A (PVA). PVA virions proved to have a peculiar combination of physicochemical properties. The intravirus coat protein (CP) subunits were shown to contain an unusually high fraction of disordered structures, whereas PVA virions had an almost normal thermal stability. Upon heating from 20uC to 55uC, the fraction of disordered structures in the intravirus CP further increased, while PVA virions remained intact at up to 55uC, after which their disruption (and DSC melting) started. We suggest that the structure of PVA virions below 55uC is stabilized by interactions between the remaining structured segments of intravirus CP. It is not improbable that the biological efficiency of PVA relies on the disordered structure of intravirus CP. Citation: Ksenofontov AL, Paalme V, Arutyunyan AM, Semenyuk PI, Fedorova NV, et al
- Добавил в систему: Ксенофонтов Александр Леонидович