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Production of biologically active human myelocytokines in plantsстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 19 июля 2013 г.
- Авторы: Zvereva A.S., Petrovskaya L.E., Rodina A.V., Frolova O.Y., Ivanov P.A., Shingarova L.N., Komarova T.V., Dorokhov Y.L., Dolgikh D.A., Kirpichnikov M.P., Atabekov J.G.
- Журнал: Biochemistry (Moscow)
- Год издания: 2009
- Издательство: Pleiades Publishing, Ltd
- Местоположение издательства: Road Town, United Kingdom
- Первая страница: 74
- Последняя страница: 11
- DOI: 10.1134/S0006297909110029
- Аннотация: Abstract—An effective system for expression of human granulocyte and granulocyte macrophage colonystimulating factors (hGCSF and hGMCSF) in Nicotiana benthamiana plants was developed using viral vector based on tobacco mosaic virus infecting cruciferous plants. The genes of target proteins were cloned into the viral vector driven by actin promoter of Arabidopsis thaliana. The expression vectors were delivered into plant cells by agroinjection. Maximal synthesis rate was detected 5 days after injection and was up to 500 and 300 mg per kg of fresh leaves for hGCSF and hGMCSF, respective ly. The yield of purified hGCSF and hGMCSF was 100 and 50 mg/kg of fresh leaves weight, respectively. Recombinant plantmade hGCSF and hGMCSF stimulated proliferation of murine bone marrow and human erythroleucosis TF1 cells, respectively, at the same rate as the commercial drugs.
- Добавил в систему: Дорохов Юрий Леонидович