Structure of antipodal cells nuclei of wheat embryo sac during programmed cell death

Doronina, T.V.; Lazareva, E.M.

Авторы: Doronina T.V., Lazareva E.M.
Журнал: Planta
Том: 254
Номер: 48
Год издания: 2021
Издательство: Springer Verlag
Местоположение издательства: Germany
DOI: 10.1007/s00425-021-03701-5
Аннотация: We studied in detail the nuclei of cells of the antipodal complex of wheat embryo sac (Triticum aestivum L.) during programmed cell death (PCD). The antipodal complex has been reported to be formed before double fertilisation of the embryo sac. Polyploidisation leads to the formation of giant polytene chromosomes in the nuclei of antipodal cells. These chromosomes are involved in secretory functions and are important for the development of cellular endosperm. Terminal deoxynucleotidyl transferase dUTP nick end labelling assay and immunodetection revealed DNA breaks in the nuclei andrelease of cytochrome c from mitochondria into the cytoplasm of antipodal cells during PCD. We used transmission electron microscopy, immunodetection and histochemistry to analyse the characteristic structural changes in the nuclei of antipodal cells during PCD. These included sequential structural changes in the nuclei containing polytene chromosomes, segregationof some components of the nucleolus into the bodies of polytene chromosomes, extrusion of nucleolar components and partsof chromosomes into the cytoplasm of antipodal cells and then into the endosperm coenocyte. The obtained results expand the understanding of the structural changes of plant cells with giant polytene chromosomes during PCD.
Добавил в систему: Доронина Татьяна Валерьевна

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Structure of antipodal cells nuclei of wheat embryo sac during programmed cell deathстатья

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