Аннотация: Many gekkonid lizards are able to produce sounds and vocalize that is considered as well- developed communication channel in this reptile group. The aim of the present study is to determine the availability of using the structure of vocal signals as additional diagnostic character in studied group. I had analyzed acoustic signals of following species of angular-toed geckoes: genus Mediodactylus Szczerbak et Golubev, 1977: M. russowii russowii, M. r. zarudnyi, M. narinensis, M. sagittifer, M. kotschyi danilevskii, M. kotschi tinensis, M.spinicauda; genus Tenuidactylus Szczerbak et Golubev, 1984: T. fedtschenkoi, T. longipes microlepis, T. caspius caspius; genus Cyrtopodion Fitzinger, 1843: C. scaber, C. agamuroides, C. gastropholis. It was showed that geckoes of genus Mediodactylus have the most highly developed vocal communication among studied species (M. russowii russowii has four types of signals). According the structure of the “territorial-breeding” calls, species of this genus were divided into two groups: 1. M. russowii russowii, M. r. zarudnyi, M. narinensis and M. sagittifer; 2. M. kotschi danilevskii, M. kotschyi tinensis, M.spinicauda. Studying of morphological and acoustic characters has allowed to conclude, that area M. russowii zarudnyi is not limited only by South-East Iran, the same form is distributed in the Central Asian sandy deserts, however early it was considered as M. r. russowii. Recently described new species M. narinensis (JERJOMTSCHENKO, ZARINENKO & PANFILIW, 1999) from Central Kyrgyzstan required to be considered as the junior synonym of M. russowii. Although the analysis of acoustic signal structure showed intra- and interspecific differences in genus Tenuidactilus in general it is homogeneous group with poorly developed vocalization. On the contrary, the genus Cyrtopodion is the combined group of species as it was shown by many researchers on the base of the analysis of morphological characters. C. agamuroides and C. gastropholis have a similar highly developed vocalization whereas acoustic signals in C. scaber are worse developed and have very different structure. The acoustic repertoire of geckoes is a good additional diagnostic feature, which is of importance for understanding of phylogenetic relationships in the studied group.