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TRANSCRIPTION FACTOR YY1 ASSOCIATES WITH PERICENTROMERIC {GAMMA}-SATELLITE DNA IN CYCLING BUT NOT IN QUIESCENT (G0) CELLSстатья Исследовательская статья
Статья опубликована в высокорейтинговом журнале
Статья опубликована в журнале из списка Web of Science и/или Scopus- Авторы: Shestakova E.A., Mansuroglu Z., Mokrani H., Ghinea N., Bonnefoy E.
- Журнал: Nucleic Acids Research
- Том: 32
- Номер: 14
- Год издания: 2004
- Издательство: Oxford University Press
- Местоположение издательства: United Kingdom
- Первая страница: 4390
- Последняя страница: 4390
- DOI: 10.1093/nar/gkh737
- Аннотация: Pericentromeric g-satellite DNA is organized in constitutiveheterochromatin structures. It comprises a234 bp sequence repeated several thousands timessurrounding the centromeric sequence of all murinechromosomes. Potential binding sites for transcriptionfactor Yin Yang 1 (YY1), a repressor or activator ofseveral cellular and viral genes, are present in pericentromericg-satelliteDNA. Usinggel retardation andchromatin immunoprecipitation, we demonstrate inthis work that YY1 specifically interacts in vitro andin vivo with g-satellite DNA. Using immunoFISH andconfocal microscopy we show that YY1 specificallyco-localizes with pericentromeric g-satellite DNAclusters organized in constitutive heterochromatinin murine L929 and 3T3 fibroblasts cell lines.Immunoelectron microscopy experiments furtherconfirmed YY1 localization in heterochromatic areas.Overall, our results demonstrate for the first time thata fraction of YY1 is directly associated with constitutiveheterochromatin structures. This associationappears physiologically relevant since the associationof YY1 with pericentromeric g-satellite DNAobserved in cycling 3T3 fibroblasts stronglydiminished in quiescent (G0) 3T3 fibroblasts. We discussthe implications of these results in the context ofheterochromatin formation as well as with regard tothe YY1-induced repression of euchromatic genes.
- Добавил в систему: Шестакова Елена Анатольевна
Прикрепленные файлы
| № | Имя | Описание | Имя файла | Размер | Добавлен |
|---|---|---|---|---|---|
| 1. | Полный текст | Исследовательская статья | Shestakova_Mansuroglu__Bonnefoy_2004.pdf | 616,0 КБ | 20 сентября 2021 [ShestakovaEAn] |