|
ИСТИНА |
Войти в систему Регистрация |
ИСТИНА ИНХС РАН |
||
Dominant role of the α-chain in rejection of tumor cells bearing a specific alloantigen in TCRα transgenic mice and in in vitro experimentsстатья Исследовательская статья
Дата последнего поиска статьи во внешних источниках: 4 марта 2022 г.
- Авторы: Zamkova Maria, Kalinina Anastasiya, Silaeva Yuliya, Persiyantseva Nadezhda, Bruter Alexandra, Deikin Alexey, Khromykh Ludmila, Kazansky Dmitry
- Журнал: Oncotarget
- Том: 10
- Номер: 47
- Год издания: 2019
- Издательство: Impact Journals
- Местоположение издательства: Albany, N.Y., United States
- Первая страница: 4808
- Последняя страница: 4821
- DOI: 10.18632/oncotarget.27093
- Аннотация: Both TCRα and TCRβ types of T-cell receptors contribute to antigen recognition. However, some TCRs have chain centricity, which means that either the α-chain or the β-chain dictates the peptide–MHC complex specificity. Most earlier reports investigated the role of well-studied β-chains in antigen recognition by TCRαβ. In a previous study, we identified TCRs specific to the H-2Kb molecule. In the present work, we generated transgenic mice carrying the α-chain of this TCR. We found that these transgenic mice rejected EL-4 tumor cells bearing alloantigen H-2Kb more effectively than wild-type mice and similarly to mice with established specific memory T cells. Moreover, we found that T cells transduced with this TCRα can inhibit EL-4 cell growth in vitro and in vivo. We also found that transgenic mice recruit fewer CD8 T cells into the peritoneal cavity at the peak of the immune response and had a significantly higher number of central memory CD8 T cells in the spleen of intact transgenic mice compared to intact wild-type control. These results indicate the ability of a single transgenic α-chain of the H-2Kb-specific TCR to determine specific recognition of the H-2Kb molecule by a repertoire of T lymphocytes and to rapidly reject H-2Kb-bearing lymphoma cells.
- Добавил в систему: Казанский Дмитрий Борисович
Прикрепленные файлы
| № | Имя | Описание | Имя файла | Размер | Добавлен |
|---|---|---|---|---|---|
| 1. | Полный текст | 27093-1034536-1-PB-Kazansky.pdf | 4,3 МБ | 12 октября 2021 [dmitry_kazansky] | |
| 2. | Результаты экспериментов | 27093-1034544-1-PB-Kazansky-Suppl.pdf | 1,9 МБ | 12 октября 2021 [dmitry_kazansky] |