Recombinant components of the EcoRII restriction-modification system: Restriction endonuclease can interact with DNA center dot RNA duplexesстатья
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Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
Аннотация:To obtain recombinant restriction endonuclease (R) and methylase (M) of the EcoRII restriction-modifcation system, bacterial strains overproducing their functional hexahistidine derivatives were constructed. Active full-length R EcoRII was produced only in cells that also expressed M.EcoRII from a multicopy plasmid. Recombinant R EcoRII bound with hybrid DNA RNA duplexes.