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Features of the population of mouse peritoneal macrophages isolated after stimulation with concanavalin A and thioglycolateстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 20 октября 2021 г.
- Авторы: Zubkova E.S., Dergilev K.V., Beloglazova I.B., Molokotina Yu D., Boldyreva M.A., Tsokolaeva Z.I., Stafeev I.S., Menshikov M.Yu, Parfyonova Ye V.
- Журнал: Bulletin of Experimental Biology and Medicine
- Том: 171
- Номер: 4
- Год издания: 2021
- Издательство: Springer Nature
- Местоположение издательства: Switzerland
- Первая страница: 532
- Последняя страница: 540
- DOI: 10.1007/s10517-021-05265-6
- Аннотация: Murine peritoneal macrophages isolated from the lavage fluid after administration of thioglycolate and concanavalin A are presented by two populations of cells of different diameters. Polarization of macrophages into a proinflammatory (M1) phenotype is accompanied by an increase in number of small cells. Macrophages obtained after administration of thioglycolate demonstrate higher tendency to anti-inflammatory (M2) phenotype, while macrophages isolated after administration of concanavalin A are committed in the proinflammatory direction. Lactate level is increased in M1 macrophages in comparison with M2 cells, which indicates predominance of glycolytic metabolism. Macrophages obtained after administration of concanavalin A have reduced mitochondrial potential, which reflects a tendency to apoptosis. Autophagy activation and inhibition neutralize the differences in pro- and anti-inflammatory properties of polarized macrophages obtained after thioglycolate administration, but have less pronounced effect on macrophages obtained after administration concanavalin A. Autophagy inhibitor increases mitochondrial potential in non-polarized macrophages obtained after administration of concanavalin A. These results demonstrate divergent properties of macrophages obtained after administration of glycolate and concanavalin A due to the difference in the mechanisms of experimental peritonitis.
- Добавил в систему: Стафеев Юрий Сергеевич