Effect of crowding and chaperones on self-association, aggregation and reconstitution of apophosphorylase bстатья

Информация о цитировании статьи получена из Scopus, Web of Science
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 23 декабря 2016 г.

Работа с статьей


[1] Effect of crowding and chaperones on self-association, aggregation and reconstitution of apophosphorylase b / N. A. Chebotareva, T. B. Eronina, S. G. Roman et al. // International Journal of Biological Macromolecules. — 2013. — Vol. 60. — P. 69–76. It was shown that the rate of reconstruction of muscle glycogen phosphorylase b (Phb) from apoenzyme and pyridoxal 5'-phosphate decreased under crowding conditions. The effect of crowding was counteracted by chaperones (alpha-crystallin and proline). Sedimentation analysis shows that crowding stimulates the formation of high-molecular-weight associates at 25 ◦C, whereas chaperones stabilize small oligomers. The study of the kinetics of apoPhb aggregation at 37 ◦C showed that the anti-aggregation activity of chaperones decreased under crowding conditions. When studying the sedimentation behavior of the mixture of apoPhb and alpha-crystallin, the complexes between unfolded apoPhb and dissociated forms of alpha-crystallin were observed. It is assumed that these complexes are responsible for realization of the chaperone-like activity of alpha-crystallin under crowding conditions. [ DOI ]

Публикация в формате сохранить в файл сохранить в файл сохранить в файл сохранить в файл сохранить в файл сохранить в файл скрыть