Effect of crowding and chaperones on self-association, aggregation and reconstitution of apophosphorylase bстатья
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Дата последнего поиска статьи во внешних источниках: 23 декабря 2016 г.
Аннотация:It was shown that the rate of reconstruction of muscle glycogen phosphorylase b (Phb) from apoenzyme
and pyridoxal 5'-phosphate decreased under crowding conditions. The effect of crowding was
counteracted by chaperones (alpha-crystallin and proline). Sedimentation analysis shows that crowding stimulates
the formation of high-molecular-weight associates at 25 ◦C, whereas chaperones stabilize small
oligomers. The study of the kinetics of apoPhb aggregation at 37 ◦C showed that the anti-aggregation
activity of chaperones decreased under crowding conditions. When studying the sedimentation behavior
of the mixture of apoPhb and alpha-crystallin, the complexes between unfolded apoPhb and dissociated
forms of alpha-crystallin were observed. It is assumed that these complexes are responsible for realization
of the chaperone-like activity of alpha-crystallin under crowding conditions.