Analysis of hybridization between tetraploid Lotus сorniculatus and diploid Lotus stepposus (Fabaceae-Loteae): morphological and molecular aspectsстатья
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Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
Аннотация:We analyzed variability of morphological
characters and genetic polymorphism of inter-simple
sequence repeat (ISSR) markers in nine natural populations
of three Lotus species from Eastern Europe, aiming to
provide insights into the nature of the species L. ucrainicus.
Nuclear ribosomal internal transcribed spacer (nrITS)
was used as an additional molecular marker for a small
subset of accessions. Analysis of variance, and principal
coordinate and principal component analyses were applied
for morphological data study. Cluster analysis [unweighted
pair-group method with arithmetic mean (UPGMA)],
principal coordinate analysis, and analysis of population
genetic structure were used for ISSR pattern study. Morphological
and genetic (ISSR, nrITS) evidence suggested
hybrid origin of L. 9 ucrainicus as a result of hybridization
between tetraploid species L. corniculatus and diploid
species L. stepposus. We conclude that L. 9 ucrainicus
may represent a case of hybrid speciation in statu nascendi,
occurring before our very eyes.