|
ИСТИНА |
Войти в систему Регистрация |
ИСТИНА ИНХС РАН |
||
Head-to-head bis-Hairpin Polyamide Minor Groove Binders and Their Conjugates with Triplex-forming Oligonucleotides: Studies of Interaction with Target Double-stranded DNAстатья
Информация о цитировании статьи получена из
Scopus,
Web of Science
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 17 января 2017 г.
- Авторы: Halby L., Ryabinin V.A., Sinyakov A.N., Novopashina D.S., Venyaminova A.G., Grokhovsky S.L., Surovaya A.N., Gursky G.V., Boutorine A.S.
- Журнал: Journal of Biomolecular Structure and Dynamics
- Том: 25
- Номер: 1
- Год издания: 2007
- Издательство: Adenine Press
- Местоположение издательства: United States
- Первая страница: 61
- Последняя страница: 76
- Аннотация: Two hairpin hexa(N-methylpyrrole)carboxamide DNA minor groove binders (MGB) were linked together via their N-termini in head-to-head orientation. Complex formation between these bis-MGB conjugates and target DNA has been studied using DNase I footprinting, circular dichroism, thermal dissociation, and molecular modeling. DNase I footprint revealed binding of these conjugates to all the sites of 492 b.p. DNA fragment containing (A/T)nXm(A/T)p sequences, where n>3, p>3; m=1,2; X = A,T,G, or C. Binding affinity depended on the sequence context of the target. CD experiments and molecular modeling showed that oligo(N-methylpyrrole)carboxamide moieties in the complex form two short antiparallel hairpins rather than a long parallel head-to-head hairpin. Binding of bis-MGB also stabilized a target duplex thermodynamically. Sequence specificity of bis-MGB/DNA binding was validated using bis-conjugates of sequence-specific hairpin (N-methylpyrrole)/ (N-methylimidazole) carboxamides.
- Добавил в систему: Гурский Георгий Валерианович
Прикрепленные файлы
| № | Имя | Описание | Имя файла | Размер | Добавлен |
|---|