Competitive Biosynthesis of Bacterial Alginate Using Azotobacter vinelandii 12 for Tissue Engineering Applications

Dudun, A.A.; Akoulina, E.A.; Zhuikov, V.A.; Makhina, T.K.; Voinova, V.V.; Belishev, N.V.; Khaydapova, D.D.; Shaitan, K.V.; Bonartseva, G.A.; Bonartsev, A.P.

Статья опубликована в высокорейтинговом журнале

Информация о цитировании статьи получена из Web of Science, Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 4 марта 2022 г.

Авторы: Dudun Andrei A., Akoulina Elizaveta A., Zhuikov Vsevolod A., Makhina Tatiana K., Voinova Vera V., Belishev Nikita V., Khaydapova Dolgor D., Shaitan Konstantin V., Bonartseva Garina A., Bonartsev Anton P.
Журнал: Polymers
Том: 14
Номер: 1
Год издания: 2022
Издательство: MDPI
Местоположение издательства: Basel, Switzerland
Первая страница: 131
Последняя страница: 152
DOI: 10.3390/polym14010131
Аннотация: This study investigated the effect of various cultivation conditions (sucrose/phosphate concentrations, aeration level) on alginate biosynthesis using the bacterial producing strain Azotobacter vinelandii 12 by the full factorial design (FFD) method and physicochemical properties (e.g., rheological properties) of the produced bacterial alginate. We demonstrated experimentally the applicability of bacterial alginate for tissue engineering (the cytotoxicity testing using mesenchymal stem cells (MSCs)). The isolated synthesis of high molecular weight (Mw) capsular alginate with a high level of acetylation (25%) was achieved by FFD method under a low sucrose concentration, an increased phosphate concentration, and a high aeration level. Testing the viscoelastic properties and cytotoxicity showed that bacterial alginate with a maximal Mw (574 kDa) formed the densest hydrogels (which demonstrated relatively low cytotoxicity for MSCs in contrast to bacterial alginate with low Mw). The obtained data have shown promising prospects in controlled biosynthesis of bacterial alginate with different physicochemical characteristics for various biomedical applications including tissue engineering.
Добавил в систему: Жуйков Всеволод Александрович

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Competitive Biosynthesis of Bacterial Alginate Using Azotobacter vinelandii 12 for Tissue Engineering Applicationsстатья

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