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Electrochemical assay of endo-depolymerase activity of cellulasesстатья
Информация о цитировании статьи получена из
Scopus,
Web of Science
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 7 октября 2013 г.
- Авторы: Skomarovsky A.A., Zorov I.N., Grishutin S.G., Markov A.V., Sinitsyn A.P., Gusakov A.V., Ryabov A.D., Gnedenko B.B.
- Журнал: Biochemistry (Moscow)
- Том: 65
- Номер: 10
- Год издания: 2000
- Издательство: Pleiades Publishing, Ltd
- Местоположение издательства: Road Town, United Kingdom
- Первая страница: 1197
- Последняя страница: 1201
- Аннотация: A method for determination of endo-1,4-beta -D-glucanase activity of cellulase samples based on the indirect measurement of decrease in viscosity of a carboxymethylcellulose solution in an electrochemical cell in the presence of an electron carrier was developed. A rotating disk electrode is used as the working electrode. When two reactions (enzymatic and electrochemical) proceeded in the cell simultaneously, the limiting diffusion current at a constant applied potential increases as the viscosity of the solution decreases. Conditions where the initial rate of change of diffusion current (dI/dt) is proportional to the enzyme concentration were found. A good correlation between the new method and a previously known viscometric method for determination of endoglucanase activity was observed.
- Добавил в систему: Гусаков Александр Васильевич