Amycolatopsis camponoti sp. nov., new tetracenomycin-producing actinomycete isolated from carpenter ant Camponotus vagus

Zakalyukina, Y.V.; Osterman, I.A.; Wolf, J.; Neumann-Schaal, M.; Nouioui, I.; Biryukov, M.V.

Авторы: Zakalyukina Yuliya V., Osterman Ilya A., Wolf Jacqueline, Neumann-Schaal Meina, Nouioui Imen, Biryukov Mikhail V.
Журнал: Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology
Том: 115
Год издания: 2022
Издательство: Springer Nature
Местоположение издательства: Switzerland
Первая страница: 533
Последняя страница: 544
DOI: 10.1007/s10482-022-01716-w
Аннотация: An actinobacterial strain A23T, isolated from adult ant Camponotus vagus collected in Ryazan region (Russia) and established as tetracenomycin X producer, was subjected to a polyphasic taxonomic study. Morphological characteristics of this strain included well-branched substrate mycelium and aerial hyphae fragmented into rod-shaped elements. Phylogenetic analyses based on 16S rRNA gene and genome sequences showed that strain A23T was most closely related to Amycolatopsis pretoriensis DSM 44654T. Average nucleotide identity and digital DNA–DNA hybridization values between the genome sequences of isolate A23T and its closest relative, Amycolatopsis pretoriensis DSM 44654T, were 39.5% and 88.6%, which were below the 70 % and 95-96% cut-off point recommended for bacterial species demarcation, respectively. The genome size of the isolate A23T was 10,560,374 bp with a DNA G+C content of 71.2%. The whole-cell hydrolysate contained meso-diaminopimelic acid and arabinose and galactose as main diagnostic sugars as well as ribose and rhamnose. It contained MK-9(H4) as the predominant mena-quinone and iso-C16:0, iso-C15:0, anteiso-C17:0 and C16:0 as the major cellular fatty acids. Diphosphatidylglycerol and phosphatidylethanolamine prevailed among phospholipids. Mycolic acids were not detected. Based on the pheno-typic, genomic and phylogenetic data, isolate A23T represents a novel species of the genus Amycolatopsis, for which the name Amycolatopsis camponoti sp. nov. is proposed, and the type strain is A23T (=DSM 111725T =VKM 2882T).
Добавил в систему: Закалюкина Юлия Владимировна

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Amycolatopsis camponoti sp. nov., new tetracenomycin-producing actinomycete isolated from carpenter ant Camponotus vagusстатья Исследовательская статья

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