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Performance improved fluorescence polarization for easy and accurate authentication of chicken adulterationстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 4 марта 2022 г.
- Авторы: Yao Li, Xu Jianguo, Qu Wei, Qiao Dongqing, Eremin Sergei A., Lu Jianfeng, Chen Wei, Qin Panzhu
- Журнал: Food Control
- Том: 133
- Год издания: 2022
- Издательство: Elsevier BV
- Местоположение издательства: Netherlands
- Первая страница: 108604
- DOI: 10.1016/j.foodcont.2021.108604
- Аннотация: Rapid, accurate, and effective authentication of unlabeled meat components in commercial meat products is of great significance for guaranteeing food safety and human health. In this research, we have constructed a single-strand binding protein (SSB)-assisted performance improved fluorescence polarization (FP) protocol for authentication of chicken component in meat products. The SSB-assisted FP strategy involves the use of fluorescein isothiocyanate (FITC)-labeled primers to perform polymerase chain reaction (PCR). In addition, FP enhancement was accomplished by mixing the amplified product with SSB. Based on the designed mechanism, the free primers in the final amplified product can be tightly bound by SSB, which greatly restricts the rotation of FITC, thereby resulting a high FP signal. In the presence of chicken DNA, the transformation of single-stranded primers to double-stranded amplicons greatly hinders the combination of SSB and primers, leading to the decrease of FP signal. This method well couples the advantages of both PCR and FP technology. Through this new strategy, as low as 0.035% (wt. %) of chicken adulteration in meat samples can be well authenticated. Moreover, this method is also available for commercial meat products. We expect this SSB-assisted FP strategy will provide new insights into the construction of versatile analysis tools in the field of food safety detection.
- Добавил в систему: Еремин Сергей Александрович
Прикрепленные файлы
| № | Имя | Описание | Имя файла | Размер | Добавлен |
|---|---|---|---|---|---|
| 1. | 22_FoodCont133_108604_FP_Chicken.pdf | 22_FoodCont133_108604_FP_Chicken.pdf | 4,4 МБ | 12 декабря 2022 [Sergei_A_EREMIN] | |
| 2. | 22_FoodCont133_108604_FP_Chicken.pdf | 22_FoodCont133_108604_FP_Chicken.pdf | 4,4 МБ | 25 февраля 2022 [Sergei_A_EREMIN] |