Diversity of Novel Uncultured Prokaryotes in Microbial Communities of the Yessentukskoye Underground Mineral Water Depositстатья
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Дата последнего поиска статьи во внешних источниках: 4 марта 2022 г.
Аннотация:Caucasian Mineral Waters is a unique territory, where various types of mineral waters with overalldaily f low over 16000 m3 are concentrated in a relatively small area. The Yessentukskoye deposit is character-ized by high diversity of water types, of which Yessentuki nos. 17 and 4 are produced in the greatest amounts.Biogeochemical activity of microorganisms inhabiting the subsurface hydrosphere is one of the proposedmechanisms responsible for the genesis of these waters. The influence of microbial communities on the qual-ity of balneological water resources is presently quite poorly studied. This is the first report on characteriza-tion of two communities inhabiting the water-bearing rocks and mineral waters of the Yessentukskoyedeposit. The 16S rRNA gene profiling of these communities revealed predominance of uncultured archaea ofthe phylum Hadarchaeota (36.6%) in the Yessentuki no. 17 water retrieved from the well 46 and of several newclasses of uncultured actinobacteria (29.4%) in the Yessentuki no. 4 water retrieved from the well 49-E. Sig-nificant differences were revealed in the structure of microbial communities inhabiting the water-bearinghorizons of these two wells having different hydrochemical characteristics. Enrichment and pure cultures ofthe microorganisms belonging to the less abundant taxa were obtained. Analysis of metadata on genomicproperties of prokaryotes of the dominant taxa, revealed in this work, indicates their ability to grow chemo-autotrophically and thus, their potential involvement in redox transformations of the water-bearing rocks andthe gas component of mineral waters.