Heterogeneity of starved yeast cells in IF1 levels suggests the role of this protein in vivoстатья
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Дата последнего поиска статьи во внешних источниках: 27 апреля 2022 г.
Аннотация:In mitochondria, a small protein IF1 suppresses the hydrolytic activity of ATP synthase and presumably prevents excessive ATPhydrolysis under conditions of energy deprivation. In yeast Saccharomyces cerevisiae, IF1 homologues are encoded by twoparalogous genes: INH1 and STF1. INH1 expression is known to aggravate the deleterious effects of mitochondrial DNA depletion.Surprisingly, no beneficial effects of INH1 and STF1 were documented for yeast so far, and the function of INH1 and STF1 in wild typecells is unclear. Here we put forward a hypothesis that INH1 and STF1 bring advantage during the fast start of proliferation afterreentry into exponential growth from post-diauxic or stationary phases. We found that yeast cells increase the concentration ofboth proteins in the post-diauxic phase. Post-diauxic phase yeast cells formed two subpopulations distinct in Inh1p and Stf1pconcentrations. Upon exit from the post-diauxic phase cells with high level of Inh1-GFP started growing earlier than cells devoid ofInh1-GFP. However, double deletion of INH1 and STF1 did not increase the lag period necessary for stationary phase yeast cells tostart growing after reinoculation into the fresh medium. These results point to a redundancy of the mechanisms preventinguncontrolled ATP hydrolysis during energy deprivation.